use of hydrocyclones for mammalian cell retention
use of hydrocyclones for mammalian cell retention
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Use of Hydrocyclones for Mammalian Cell Retention ...

A hydrocyclone with a volume of 2.56 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply).

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Use of Hydrocyclones for Mammalian Cell Retention ...

Request PDF Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell ...

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The Use of Hydrocyclones for Mammalian Cell Retention in ...

(2001) The Use of Hydrocyclones for Mammalian Cell Retention in Perfusion Bioreactors. In: Lindner-Olsson E., Chatzissavidou N., Lüllau E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1.

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Use of Hydrocyclones for Mammalian Cell Retention ...

Aug 01, 2006  Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) Elsayed, E. A.; Medronho, R. A.; Wagner, R.; Deckwer, W.‐D. 2006-08-01 00:00:00 A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell ...

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Hydrocyclones as cell retention device for CHO perfusion ...

In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the

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Hydrocyclones as cell retention device for CHO perfusion ...

Mar 17, 2020  Hydrocyclones as cell retention device for CHO perfusion processes in single‐use bioreactors ... (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an ...

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Hydrocyclones as cell retention device for CHO perfusion ...

Mar 17, 2020  Hydrocyclones as cell retention device for CHO perfusion processes in single-use bioreactors. Bettinardi IW(1), Castan A(2), Medronho RA(3), Castilho LR(1). Author information: (1)Federal University of Rio de Janeiro (UFRJ), COPPE/PEQ, Cell Culture Engineering Laboratory (LECC), Rio de Janeiro/RJ, Brazil.

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Hydrocyclones as cell retention device for CHO perfusion ...

Request PDF Hydrocyclones as cell retention device for CHO perfusion processes in single‐use bioreactors In this study, a hydrocyclone (HC) especially designed for mammalian cell separation ...

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(PDF) Separation of CHO cells using hydrocyclones ...

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) By R. Medronho. A model for performance prediction of hydrocyclones. By R. Medronho. A new method for yeast recovery in batch ethanol fermentations: Filter aid filtration followed by separation of yeast from filter aid using hydrocyclones.

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Application of Hydrocyclone for Cell Separation in ...

mammalian cell culture. Generally, large-scale commercial mammalian cell cultivations are performed in batch, fed-batch and perfusion processes. Perfusion systems involve cell retention in the bioreactor with continuous exchange of the medium and simultaneous removal of the byproducts. Perfusion cultivations have the advantage of continuously

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The Potential of Hydrocyclone Application for Mammalian ...

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) E. Elsayed , R. Medronho , R. Wagner , W. Deckwer Biology

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(PDF) Separation of CHO cells using hydrocyclones ...

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) By R. Medronho. A model for performance prediction of hydrocyclones. By R. Medronho. A new method for yeast recovery in batch ethanol fermentations: Filter aid filtration followed by separation of yeast from filter aid using hydrocyclones.

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Separation of CHO cells using hydrocyclones SpringerLink

Nov 14, 2007  Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design. It was shown that cell separation efficiencies for cultures of the wild-type ...

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Separation of CHO cells using hydrocyclones. - Abstract ...

Nov 14, 2007  Jockwer A, Medronho RA, Wagner R, Anspach FB, Deckwer WD (2001) The use of hydrocyclones for mammalian cell retention in perfusion bioreactors. In: Olsson EL, Chatzissavidou N, Lüllau E (eds) Animal cell technology: from target to

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[PDF] Numerical simulation of hydrocyclones for cell ...

Numerical simulation of hydrocyclones aiming at investigating the separation of microorganisms and mammalian cells was performed using Computational Fluid Dynamics (CFD). The turbulence model used in the 2d- axisymmetric calculations was the Reynolds Stress Model (RSM), in order to take into account the high swirl effects that occur in this type of equipment, which induce anisotropic turbulence.

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US6878545B2 - Method for separating viable cells from cell ...

Replacing known separation and cell-retention systems by hydrocyclones reduces the use of capital and the operating costs. The method according to the invention using hydrocyclones can, in particular, be used for cell retention in perfusion cultures. Literature Sources. Bradley, D. (1965) The Hydrocyclone. Pergamon Press, Oxford.

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Separation of yeast from alcoholic fermentation in small ...

Mar 05, 2012  Use of hydrocyclones for mammalian cell retention: separation efficiency and cell viability [Part 1] Engineering Life Science , 6 ( 2006 ) , pp. 347 -

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Bioreactor Design and Scale-Up Springer for Research ...

Jockwer A, Medronho RA, Wagner R, Anspach FB, Deckwer W-D (2001) The use of hydrocyclones for mammalian cell retention in perfusion bioreactors. In: Lindner-Olsson E, Chatzissavidou N, Lüllau E (eds) Animal Cell Technology: From Target to Market, pp 301–305. Kluwer, Dordrecht Google Scholar

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Perfusion bioreactor with TFF cell retention Cytiva

This study demonstrates perfusion culture of an antibody-producing CHO cell line, with tangential flow filtration (TFF) as cell retention method. The setup includes a single-use stirred-tank bioreactor system, Xcellerex XDR-10, and a single-use hollow fiber filter cartridge as cell retention device.

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EP1280885B1 - Method for separating viable mammal or ...

EP1280885B1 EP20010927844 EP01927844A EP1280885B1 EP 1280885 B1 EP1280885 B1 EP 1280885B1 EP 20010927844 EP20010927844 EP 20010927844 EP 01927844 A EP01927844 A EP 01927844A EP 1280885 B1 EP1280885 B1 EP 1280885B1 Authority EP European Patent Office Prior art keywords cells hydrocyclone outlet diameter hydrocyclones Prior art date 2000-05-11 Legal status

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"Hydrocyclones for single-use perfusion application" by ...

Said that, hydrocyclones can be produced by 3D printing, making them a promising alternative for the integration of cell retention devices in single-use bioreactor bags. The performance of hydrocyclones also depends on the attachment configuration to the bioreactor and cell concentration of

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A direct computer control concept for mammalian cell ...

In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems.

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R. Medronho - Academia

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) more. by R. Medronho. Publication Date: 2006 Publication Name: Engineering in Life Sciences. Research Interests: Engineering, Technology, Cell Viability, and Cyclones.

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Application of Hydrocyclone for Cell Separation in ...

mammalian cell culture. Generally, large-scale commercial mammalian cell cultivations are performed in batch, fed-batch and perfusion processes. Perfusion systems involve cell retention in the bioreactor with continuous exchange of the medium and simultaneous removal of the byproducts. Perfusion cultivations have the advantage of continuously

More

Separation of CHO cells using hydrocyclones. - Abstract ...

Nov 14, 2007  Jockwer A, Medronho RA, Wagner R, Anspach FB, Deckwer WD (2001) The use of hydrocyclones for mammalian cell retention in perfusion bioreactors. In: Olsson EL, Chatzissavidou N, Lüllau E (eds) Animal cell technology: from target to

More

"Hydrocyclones for single-use perfusion application" by ...

Said that, hydrocyclones can be produced by 3D printing, making them a promising alternative for the integration of cell retention devices in single-use bioreactor bags. The performance of hydrocyclones also depends on the attachment configuration to the bioreactor and cell concentration of

More

EP1280885B1 - Method for separating viable mammal or ...

EP1280885B1 EP20010927844 EP01927844A EP1280885B1 EP 1280885 B1 EP1280885 B1 EP 1280885B1 EP 20010927844 EP20010927844 EP 20010927844 EP 01927844 A EP01927844 A EP 01927844A EP 1280885 B1 EP1280885 B1 EP 1280885B1 Authority EP European Patent Office Prior art keywords cells hydrocyclone outlet diameter hydrocyclones Prior art date 2000-05-11 Legal status

More

US6878545B2 - Method for separating viable cells from cell ...

Replacing known separation and cell-retention systems by hydrocyclones reduces the use of capital and the operating costs. The method according to the invention using hydrocyclones can, in particular, be used for cell retention in perfusion cultures. Literature Sources. Bradley, D. (1965) The Hydrocyclone. Pergamon Press, Oxford.

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R. Medronho - Academia

Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) more. by R. Medronho. Publication Date: 2006 Publication Name: Engineering in Life Sciences. Research Interests: Engineering, Technology, Cell Viability, and Cyclones.

More

Bioreactor Design and Scale-Up Springer for Research ...

Jockwer A, Medronho RA, Wagner R, Anspach FB, Deckwer W-D (2001) The use of hydrocyclones for mammalian cell retention in perfusion bioreactors. In: Lindner-Olsson E, Chatzissavidou N, Lüllau E (eds) Animal Cell Technology: From Target to Market, pp 301–305. Kluwer, Dordrecht Google Scholar

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A direct computer control concept for mammalian cell ...

In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems.

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CELL RETENTION DEVICE AND METHOD - Boehringer Ingelheim ...

The subject technology relates to a cell retention device and method for use in a perfusion cell culture system where the cell retention device includes a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 μm and having the ability to operate under perfusion cell culture conditions for up to 35 days without being clogged or losing its product sieving ability by ...

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types of hydrocyclones in grinding plant pdf

The sizing and selection of hydrocyclones889 Кб. Although the method for determining the proper size and selection of hydrocyclones in this paper has proven to be quite accurateMular, A. L. and Jull, N.A., 1978, The Selection of Cyclone Classifiers, Pumps and Pump Boxes for Grinding Circuits, Mineral Processing Plant Design, AIME, New York.

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Animal Cell Technology: From Target to Market: Proceedings ...

Dec 06, 2012  The Use of Hydrocyclones for Mammalian Cell Retention in Perfusion . 301: A Study of Critical Parameters Using CHO Cell . 318: Growth of HighFive Cells on CultispherS Microbarriers . 332: Genzel Y Voges L and Reichl U . 344: Scale up of . 358: A New Method for Solubilization of Lipophilic Substances . 372:

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Acoustic Settler, Centrifuge, Hydrocyclone - Animal Cell ...

Hence higher acceleration provokes higher damage. The cell damage can be significantly reduced by using the centrifuge in intermittent mode, i.e. several minutes per hour or so (Johnson et al. 1996). Hydrocyclone. A detailed description of the hydrocyclone for mammalian cell culture can be found in Castilho's review (Castilho and Medronho 2002).

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Introduction to Sample Preparation: Immunofluorescence ...

Slides and coverslips are the easiest thing to use for most fixed samples. (Obviously living samples are completely different and here are some suggestions for live cell imaging). Tissue culture cells can be grown directly on coverslips. In general you should use #1.5 coverslips as most microscope objectives are designed to work optimally with ...

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Limited Use of Centritech Lab II Centrifuge in Perfusion ...

maintenance of optimal cell growth conditions, continuous product removal and medium exchange with simultaneous withdrawal of toxic metabolites must be ensured. Therefore, application of the perfusion mode to a mammalian cell culture process depends on the use of efficient and desirable cell retention

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